Isotype Control Antibodies | Thermo Fisher Scientific - TW

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Isotype controls are primary antibodies that lack specificity to the target, but match the class and type of the primary antibody used in the application. PopularApplications&TechniquesShopAllProductsServicesSupport Signin QuickOrder     Search ThermoFisherScientific SearchAll Search Home›LifeSciences›Antibodies›PrimaryAntibodies›ControlAntibodies›IsotypeControlAntibodiesIsotypeControlAntibodiesSeeNavigation‹ControlAntibodiesIsotypeControlAntibodies ›LoadingControlAntibodies ›Isotypecontrolsareprimaryantibodiesthatlackspecificitytothetarget,butmatchtheclassandtypeoftheprimaryantibodyusedintheapplication.Isotypecontrolsareusedasnegativecontrolstohelpdifferentiatenon-specificbackgroundsignalfromspecificantibodysignal.Dependingupontheisotypeoftheprimaryantibodyusedfordetectionandthetargetcelltypesinvolved,backgroundsignalmaybeasignificantissueinvariousexperiments.Causesofbackgroundstainingassociatedwithprimaryantibodiesinclude:bindingtoFcreceptorsontargetcellsnonspecificantibodyinteractionswithcellularproteins,carbohydrates,andlipidscellularautofluorescenceSelectinganisotypecontrolIsotypecontrolsshouldbematchedtotheprimaryantibody’shostspeciesandclass,includinglightchains(e.g.,IgG2Lambda,IgA1Kappa).Ifusingdirectlylabeledprimaryantibodies,itisimportanttousethesameconjugatetoavoiddifferencesindetection.ApplicationsforisotypecontrolsAlthoughisotypecontrolsaremainlyusedinflowcytometry,theycanbeusedasstandardblockingagentsandproteincoatingagentsforotherapplicationsincludingimmunofluorescence,immunocytochemistry,westernblotting,andELISA.Viewallisotypecontrols Resources AntibodiesLearningCenterOverviewofWesternBlottingFluorescentWesternBlottingAntibodiesforFlowCytometryFlowCytometryResourceCenter Signin Don'thaveanaccount? 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