精油抗第一型單純皰疹病毒研究__臺灣博碩士論文知識加值系統

植物中有許多成份或者其代謝物已有研究說明其抗皰疹病毒的特質，以天然植物為基礎而製成的精油亦具備抗病毒活性。

現今所使用的抗病毒藥物多針對病毒複製的步驟來加以 ... 資料載入處理中... 跳到主要內容 臺灣博碩士論文加值系統 ::: 網站導覽| 首頁| 關於本站| 聯絡我們| 國圖首頁| 常見問題| 操作說明 English |FB專頁 |Mobile 免費會員 登入| 註冊 功能切換導覽列 (167.99.71.17)您好！臺灣時間：2022/09/2108:35 字體大小：       ::: 詳目顯示 recordfocus 第1筆/ 共1筆  /1頁 論文基本資料 摘要 外文摘要 目次 參考文獻 紙本論文 QRCode 本論文永久網址: 複製永久網址Twitter研究生:莊和鑫研究生(外文):He-ShingChuang論文名稱:精油抗第一型單純皰疹病毒研究論文名稱(外文):TheeffectsofessentialoilonHerpessimplexvirustypeI指導教授:蔡英傑指導教授(外文):Ying-ChiehTsai學位類別:碩士校院名稱:國立陽明大學系所名稱:生化暨分子生物研究所學門:生命科學學門學類:生物化學學類論文種類:學術論文論文出版年:2009畢業學年度:97語文別:中文論文頁數:77中文關鍵詞:病毒、精油、皰疹、治療外文關鍵詞:herpessimplexvirus、essentialoil、treatment相關次數: 被引用:0點閱:587評分:下載:0書目收藏:0 單純皰疹病毒是傳染力極高的病原體，在世界各國都具有相當的盛行率，也是十分嚴重的衛生問題。

其最具有威脅性的要點就是能夠潛伏在人類的三叉神經元。

當細胞受到感染之後，病毒會從週邊神經系統沿著突觸到中央神經元潛伏，每當宿主免疫力下降時，病毒便會再次甦醒，引起諸多病徵，如唇皰疹、皰疹性角膜炎、鼻炎、腦髓炎、甚至是生殖器的黏膜部位等。

植物中有許多成份或者其代謝物已有研究說明其抗皰疹病毒的特質，以天然植物為基礎而製成的精油亦具備抗病毒活性。

現今所使用的抗病毒藥物多針對病毒複製的步驟來加以抑制，長久下來，已衍生許多具備抗藥性的病毒株，往往為了達到更佳的治療效果加重給藥劑量，因藥物副作用而就診的病例也越來越多。

因此，找尋新的抗病毒方法以及開發新藥物已是迫在眉睫，此時，由天然界植物當中所找到抗病毒的有效成份可成為替代的方案。

在本實驗中，首先以不同精油用不同的抗病毒模式進行篩選，當中找到了左手香精油在病毒前處理的步驟對抗皰疹病毒的活性相當優異，而其主要成份香芹酚亦有不錯的抗病毒功效，為了要進一步探討此抗病毒活性是否與其結構相關，因此針對香芹酚各官能基做不同改變，找尋適合的化合物進行結構活性分析。

從結構活性的分析比對中可以發現，在香芹酚上的氫氧基對於抗病毒的效果佔有決定性的影響，除了氫氧基的有無外，氫氧基的位置也會影響抗病毒活性的差異。

由電子顯微鏡的直接觀察可以發現，經過香芹酚處理後的病毒結構明顯被破壞，顯示香芹酚能夠直接對病毒體本身產生抑制的效果，在往後亦可成為對抗皰疹系統中的一環，成為有效的抗病毒藥劑，預防病毒的入侵。

Herpessimplexviruses(HSV)arewidespreadpathogensthatmakeseriouspublichealthproblemthroughouttheworld.AfterHSVinfection,thevirusthentransportthroughretrogradeaxonaltoneuronsinsensoryganglion.Duringtheperiodsofinfection,theinfectedcellundergoviruslyticpathwayorlatentpathway.Herpessimplexvirusproduceslesionsmostlyinaroundoralcavity,eyes,noseandgenitalmucousmembranes.Severalantiherpicexperimentsonplantextractandthederivedmetaboliteshavebeenreported.Essentialoilofplantextractmayexhibitedvirucidalactivitiesthatmaybeanidealmicrobicides.Thecurrentlyantiviraldrugwhichtreatmentherpesvirusproducetoxiceffect,drugresistanceproblem.Therefore,newmethodsanddrugtotreatviruslesionshouldbedevelopmentsuchastopicalapplicationandthenatureplantextract.Intheexperiment,Iscreenedtheantiviralactivitiesofseveralcompoundsfromessentialoilsbydifferentantiviralmodesderivedfromplaqueassay.Plectranthusamboinicusandthemajorcomponent,carvacrol,exhibitgreatactivitiesforantivirusbyviruspretreatmentmode.Thestructuralrequirementsfortheactivitiesofcarvacrolweredeterminedbycomparisontostructurallyrelatedcompounds.Here,Iexaminedtheeffectofthehydroxylgroupofcarvacrolandthymol,oneofcarvacrol-relatedcompounds,onantiviralactivitiesandtryingtofindoutthemechanismoftheantiviralactivities.Electronmicroscopic(EM)studyshowedthatthepurifiedvirionsexposedtocarvacrolortherelatedcompoundsweredamaged.TheresultssuggestthatcarvacrolcouldinactivateHSVvirionsdirectlyandappearstobeapromisingcandidateforusinginamicrobicidetoreduceHSVtransmission. 目錄一、緒論………………………………………………………………………………………………...61.單純皰疹病毒91.1.病毒構造101.2.單純皰疹病毒生命週期101.3.流行病學研究131.3.1.病理原因131.3.2.流行病學研究142.治療152.1.抗皰疹類藥物162.1.1.以抑制病毒生活史為對象162.1.2.以抑制細胞內因子為對象172.1.3.局部塗抹以預防病毒入侵182.2.抗藥性單純皰疹病毒202.2.1.抗藥性的機制202.2.2.抗藥性病毒流行病學研究213.天然植物抗病毒活性213.1.植物精油223.2.左手香精油244.研究動機255.實驗設計25二、材料與實驗方法281.實驗材料暨試劑配製281.1.實驗材料281.2.試劑配製281.2.1.DMEM細胞培養液281.2.2.XTT試劑291.2.3.病毒培養膠291.2.4.結晶紫染劑291.2.5.TNE緩衝液301.2.6.磷酸鎢染劑301.2.7.磷酸鹽緩衝溶液301.2.8.Karnovsky固定劑(Karnovsky’sfixative)301.3.儀器使用312.細胞培養313.病毒培養324.病毒純化325.氣相層析質譜儀分析(GC-MS)336.細胞毒性測試347.以空斑計數試驗進行病毒定量358.以組織細胞半數感染力價(TCID50)進行病毒定量369.抗病毒模式建立3610.化合物在不同時間點下對抗病毒活性的影響3811.化合物在不同濃度下對抗病毒活性的影響3812.相似化合物對抗病毒活性影響3913.穿透式電子顯微鏡檢驗39三、實驗結果411.單純�Q疹病毒感染細胞後的細胞型態變化412.不同精油內主要成份對抗病毒的影響413.左手香精油成份分析424.香芹酚(carvacrol)以及百里酚(thymol)的細胞毒性425.百里酚以及香芹酚在各種抗病毒模式下活性的表現436.給予不同抑制時間對於病毒量的影響447.香芹酚及百里酚直接抑制病毒體(virion)448.分子結構和抗病毒之間的關聯(structure-activityrelationship)459.香芹酚及百里酚對病毒所造成結構上的影響47四、討論491.植物精油抗病毒能力探討492.香芹酚活性比較503.香芹酚相似物及抗病毒關聯性514.抑制病毒的可能機轉53五、參考文獻55表目錄表一、對抗EV71腸病毒有效的數種精油與其主要成份。

61表二、左手香的精油成份分析。

62表三、carvacrol相似物活性比較。

63圖目錄圖一、第一型單純�Q疹病毒結構卡通圖64圖二、病毒成熟後的外吐模式65圖三、病毒感染模式設計簡圖66圖四、單純�Q疹病毒感染細胞後的細胞型態變化。

67圖五、各種精油主要成份對抗�Q疹病毒研究。

68圖六、細胞毒性測試。

69圖六、香芹酚及百里酚在不同抗病毒模式下所產生的活性。

70圖七、carvacrol以及thymol在不同時間點下對於單純�Q疹病毒的影響。

71圖八、carvacrol以及thymol在不同濃度下對於單純�Q疹病毒的影響。

72圖九、相似物結構。

73圖十、carvacrol相似物甲基、異丙基比較結果。

74圖十一、carvacrol相似物中，氫氧基改變後對於抗病毒活性的影響。

76圖十二、carvacrol相似物中，疏水端長度的比較。

75圖十三、以電子顯微鏡觀察�Q疹病毒受到化合物處理後產生的現象。

77圖十三、以電子顯微鏡觀察�Q疹病毒受到化合物處理後產生的現象。

78 1.ShuklaD,SpearPG:Herpesvirusesandheparansulfate:anintimaterelationshipinaidofviralentry.JClinInvest2001,108:503-510.2.HamiltonJB:NotesonFormsofKeratitisPresumablyDuetotheVirusofHerpesSimplex.BrJOphthalmol1943,27:80-87.3.XuF,SternbergMR,KottiriBJ,McQuillanGM,LeeFK,NahmiasAJ,BermanSM,MarkowitzLE:Trendsinherpessimplexvirustype1andtype2seroprevalenceintheUnitedStates.JAMA2006,296:964-973.4.BoehmerPE,NimonkarAV:Herpesvirusreplication.IUBMBLife2003,55:13-22.5.SedyJR,SpearPG,WareCF:Cross-regulationbetweenherpesvirusesandtheTNFsuperfamilymembers.NatRevImmunol2008,8:861-873.6.ArduinoPG,PorterSR:HerpesSimplexVirusType1infection:overviewonrelevantclinico-pathologicalfeatures.JOralPatholMed2008,37:107-121.7.NguyenML,BlahoJA:Apoptosisduringherpessimplexvirusinfection.AdvVirusRes2007,69:67-97.8.HerpessimplexvirusResearchonWorldWideWebURL:http://darwin.bio.uci.edu/~faculty/wagner/hsv4f.html9.MettenleiterTC,KluppBG,GranzowH:Herpesvirusassembly:ataleoftwomembranes.CurrOpinMicrobiol2006,9:423-429.10.LiashkovichI,HafeziW,KuhnJE,OberleithnerH,KramerA,ShahinV:ExceptionalmechanicalandstructuralstabilityofHSV-1unveiledwithfluidatomicforcemicroscopy.JCellSci2008,121:2287-2292.11.HochbergE,BeckerY:Adsorption,penetrationanduncoatingofherpessimplexvirus.JGenVirol1968,2:231-241.12.MettenleiterTC:Herpesvirusassemblyandegress.JVirol2002,76:1537-1547.13.PertelPE,FridbergA,ParishML,SpearPG:CellfusioninducedbyherpessimplexvirusglycoproteinsgB,gD,andgH-gLrequiresagDreceptorbutnotnecessarilyheparansulfate.Virology2001,279:313-324.14.SpearPG,LongneckerR:Herpesvirusentry:anupdate.JVirol2003,77:10179-10185.15.QuinnJP,DalzielRG,NashAA:Herpesviruslatencyinsensoryganglia--acomparisonwithendogenousneuronalgeneexpression.ProgNeurobiol2000,60:167-179.16.KnezJ,BilanPT,CaponeJP:Asingleaminoacidsubstitutioninherpessimplexvirustype1VP16inhibitsbindingtothevirionhostshutoffproteinandisincompatiblewithvirusgrowth.JVirol2003,77:2892-2902.17.LoiaconoCM,TausNS,MitchellWJ:Theherpessimplexvirustype1ICP0promoterisactivatedbyviralreactivationstimuliintrigeminalganglianeuronsoftransgenicmice.JNeurovirol2003,9:336-345.18.EfstathiouS,PrestonCM:Towardsanunderstandingofthemolecularbasisofherpessimplexviruslatency.VirusRes2005,111:108-119.19.StanberryLR,CunninghamAL,MindelA,ScottLL,SpruanceSL,AokiFY,LaceyCJ:Prospectsforcontrolofherpessimplexvirusdiseasethroughimmunization.ClinInfectDis2000,30:549-566.20.KameyamaT,SujakuC,YamamotoS,HwangCB,ShillitoeEJ:Sheddingofherpessimplexvirustype1intosaliva.JOralPathol1988,17:478-481.21.SpruanceSL,EvansTG,McKeoughMB,ThaiL,AraneoBA,DaynesRA,MishkinEM,AbramovitzAS:Th1/Th2-likeimmunityandresistancetoherpessimplexlabialis.AntiviralRes1995,28:39-55.22.StockC,Guillen-GrimaF,deMendozaJH,Marin-FernandezB,Aguinaga-OntosoI,KramerA:Riskfactorsofherpessimplextype1(HSV-1)infectionandlifestylefactorsassociatedwithHSV-1manifestations.EurJEpidemiol2001,17:885-890.23.SmithJS,RobinsonNJ:Age-specificprevalenceofinfectionwithherpessimplexvirustypes2and1:aglobalreview.JInfectDis2002,186Suppl1:S3-28.24.SchangLM:Herpessimplexvirusesinantiviraldrugdiscovery.CurrPharmDes2006,12:1357-1370.25.KaufmanHE,NesburnAB,MaloneyED:IDUtherapyofherpessimplex.ArchOphthalmol1962,67:583-591.26.ElionGB,FurmanPA,FyfeJA,deMirandaP,BeauchampL,SchaefferHJ:Selectivityofactionofanantiherpeticagent,9-(2-hydroxyethoxymethyl)guanine.ProcNatlAcadSciUSA1977,74:5716-5720.27.DeClercqE:Antiviraldrugsincurrentclinicaluse.JClinVirol2004,30:115-133.28.BrideauRJ,KnechtelML,HuangA,VaillancourtVA,VeraEE,OienNL,HopkinsTA,WieberJL,WilkinsonKF,RushBD,etal.:Broad-spectrumantiviralactivityofPNU-183792,a4-oxo-dihydroquinoline,againsthumanandanimalherpesviruses.AntiviralRes2002,54:19-28.29.KleymannG,FischerR,BetzUA,HendrixM,BenderW,SchneiderU,HandkeG,EckenbergP,HewlettG,PevznerV,etal.:Newhelicase-primaseinhibitorsasdrugcandidatesforthetreatmentofherpessimplexdisease.NatMed2002,8:392-398.30.NewcombWW,BrownJC:InhibitionofherpessimplexvirusreplicationbyWAY-150138:assemblyofcapsidsdepletedoftheportalandterminaseproteinsinvolvedinDNAencapsidation.JVirol2002,76:10084-10088.31.CoenDM,SchafferPA:Antiherpesvirusdrugs:apromisingspectrumofnewdrugsanddrugtargets.NatRevDrugDiscov2003,2:278-288.32.SchangLM:Effectsofpharmacologicalcyclin-dependentkinaseinhibitorsonviraltranscriptionandreplication.BiochimBiophysActa2004,1697:197-209.33.SchangLM:Thecellcycle,cyclin-dependentkinases,andviralinfections:newhorizonsandunexpectedconnections.ProgCellCycleRes2003,5:103-124.34.WaldA,LangenbergAG,LinkK,IzuAE,AshleyR,WarrenT,TyringS,DouglasJM,Jr.,CoreyL:Effectofcondomsonreducingthetransmissionofherpessimplexvirustype2frommentowomen.JAMA2001,285:3100-3106.35.HeroldBC,KirkpatrickR,MarcellinoD,TravelsteadA,PilipenkoV,KrasaH,BremerJ,DongLJ,CooperMD:Bilesalts:naturaldetergentsforthepreventionofsexuallytransmitteddiseases.AntimicrobAgentsChemother1999,43:745-751.36.TuyamaAC,CheshenkoN,CarlucciMJ,LiJH,GoldbergCL,WallerDP,AndersonRA,ProfyAT,KlotmanME,KellerMJ,etal.:ACIDFORMinactivatesherpessimplexvirusandpreventsgenitalherpesinamousemodel:optimalcandidateformicrobicidecombinations.JInfectDis2006,194:795-803.37.YasinB,WangW,PangM,CheshenkoN,HongT,WaringAJ,HeroldBC,WagarEA,LehrerRI:Thetadefensinsprotectcellsfrominfectionbyherpessimplexvirusbyinhibitingviraladhesionandentry.JVirol2004,78:5147-5156.38.YangD,ChertovO,OppenheimJJ:Participationofmammaliandefensinsandcathelicidinsinanti-microbialimmunity:receptorsandactivitiesofhumandefensinsandcathelicidin(LL-37).JLeukocBiol2001,69:691-697.39.KellerMJ,TuyamaA,CarlucciMJ,HeroldBC:Topicalmicrobicidesforthepreventionofgenitalherpesinfection.JAntimicrobChemother2005,55:420-423.40.JingB,JanoutV,HeroldBC,KlotmanME,HealdT,RegenSL:Persulfatedmolecularumbrellasasanti-HIVandanti-HSVagents.JAmChemSoc2004,126:15930-15931.41.SmithKO:SomeBiologicAspectsofHerpesvirus-CellInteractionsinthePresenceof5-Iodo,2-Desoxyuridine(Idu).DemonstrationofaCytotoxicEffectbyHerpesvirus.JImmunol1963,91:582-590.42.SibrackCD,GutmanLT,WilfertCM,McLarenC,StClairMH,KellerPM,BarryDW:Pathogenicityofacyclovir-resistantherpessimplexvirustype1fromanimmunodeficientchild.JInfectDis1982,146:673-682.43.MorfinF,ThouvenotD:Herpessimplexvirusresistancetoantiviraldrugs.JClinVirol2003,26:29-37.44.LarderBA,ChengYC,DarbyG:Characterizationofabnormalthymidinekinasesinducedbydrug-resistantstrainsofherpessimplexvirustype1.JGenVirol1983,64Pt3:523-532.45.FieldHJ:Herpessimplexvirusantiviraldrugresistance--currenttrendsandfutureprospects.JClinVirol2001,21:261-269.46.ChristophersJ,ClaytonJ,CraskeJ,WardR,CollinsP,TrowbridgeM,DarbyG:SurveyofresistanceofherpessimplexvirustoacyclovirinnorthwestEngland.AntimicrobAgentsChemother1998,42:868-872.47.NugierF,ColinJN,AymardM,LangloisM:Occurrenceandcharacterizationofacyclovir-resistantherpessimplexvirusisolates:reportonatwo-yearsensitivityscreeningsurvey.JMedVirol1992,36:1-12.48.FifeKH,CrumpackerCS,MertzGJ,HillEL,BooneGS:Recurrenceandresistancepatternsofherpessimplexvirusfollowingcessationof>or=6yearsofchronicsuppressionwithacyclovir.AcyclovirStudyGroup.JInfectDis1994,169:1338-1341.49.StrausSE,TakiffHE,SeidlinM,BachrachS,LiningerL,DiGiovannaJJ,WesternKA,SmithHA,LehrmanSN,Creagh-KirkT,etal.:Suppressionoffrequentlyrecurringgenitalherpes.Aplacebo-controlleddouble-blindtrialoforalacyclovir.NEnglJMed1984,310:1545-1550.50.HardyWD:Foscarnettreatmentofacyclovir-resistantherpessimplexvirusinfectioninpatientswithacquiredimmunodeficiencysyndrome:preliminaryresultsofacontrolled,randomized,regimen-comparativetrial.AmJMed1992,92:30S-35S.51.SafrinS,CrumpackerC,ChatisP,DavisR,HafnerR,RushJ,KesslerHA,LandryB,MillsJ:Acontrolledtrialcomparingfoscarnetwithvidarabineforacyclovir-resistantmucocutaneousherpessimplexintheacquiredimmunodeficiencysyndrome.TheAIDSClinicalTrialsGroup.NEnglJMed1991,325:551-555.52.JassimSA,NajiMA:Novelantiviralagents:amedicinalplantperspective.JApplMicrobiol2003,95:412-427.53.WoodHC,ReichutET:NoteontheActionupontheCirculationofcertainVolatileOils.JPhysiol1880,2:446.54.LukhobaCW,SimmondsMS,PatonAJ:Plectranthus:areviewofethnobotanicaluses.JEthnopharmacol2006,103:1-24.55.MortonJF:Countryborage(ColeusamboinicusLour.):apotentflavoringandmedicinalplant.JournalofHerbs,Spices&MedicinalPlants1992,1:77-90.56.ChangSL,ChangYC,YangCH,HongHS:AllergiccontactdermatitistoPlectranthusamboinicusmasqueradingaschroniclegulcer.ContactDermatitis2005,53:356-357.57.PeriyanayagamK,NirmalaDeviK,SuseelaL,UmaA,IsmailM:InvivoantimalarialactivityofleavesofPlectranthusamboinicus(lour)sprengonPlasmodiumbergheiyoelii.JCommunDis2008,40:121-125.58.ChangJM,ChengCM,HungLM,ChungYS,WuRY:PotentialUseofPlectranthusamboinicusintheTreatmentofRheumatoidArthritis.EvidBasedComplementAlternatMed2007.59.IsaacsCE,WenGY,XuW,JiaJH,RohanL,CorboC,DiMaggioV,JenkinsEC,Jr.,HillierS:Epigallocatechingallateinactivatesclinicalisolatesofherpessimplexvirus.AntimicrobAgentsChemother2008,52:962-970.60.SchnitzlerP,SchuhmacherA,AstaniA,ReichlingJ:Melissaofficinalisoilaffectsinfectivityofenvelopedherpesviruses.Phytomedicine2008,15:734-740.61.AguilarJS,HeldKS,WagnerEK:Herpessimplexvirustype1showsmultipleinteractionswithsulfonatedcompoundsatbinding,penetration,andcell-to-cellpassage.VirusGenes2007,34:241-248.62.CheshenkoN,DelRosarioB,WodaC,MarcellinoD,SatlinLM,HeroldBC:Herpessimplexvirustriggersactivationofcalcium-signalingpathways.JCellBiol2003,163:283-293.63.KochC,ReichlingJ,SchneeleJ,SchnitzlerP:Inhibitoryeffectofessentialoilsagainstherpessimplexvirustype2.Phytomedicine2008,15:71-78.64.SchnitzlerP,KochC,ReichlingJ:Susceptibilityofdrug-resistantclinicalherpessimplexvirustype1strainstoessentialoilsofginger,thyme,hyssop,andsandalwood.AntimicrobAgentsChemother2007,51:1859-1862.65.VerzeraA,TrozziA,ZappalaM,CondursoC,CotroneoA:EssentialoilcompositionofCitrusmeyeriiY.Tan.andCitrusmedicaL.cv.Diamanteandtheirlemonhybrids.JAgricFoodChem2005,53:4890-4894.66.AllahverdiyevA,DuranN,OzguvenM,KoltasS:AntiviralactivityofthevolatileoilsofMelissaofficinalisL.againstHerpessimplexvirustype-2.Phytomedicine2004,11:657-661.67.FaithSA,SweetTJ,BaileyE,BoothT,DochertyJJ:Resveratrolsuppressesnuclearfactor-kappaBinherpessimplexvirusinfectedcells.AntiviralRes2006,72:242-251.68.MericliFI,TankerM:Thevolatileoilsofsomeendemicthymusspeciesgrowinginsouthernanatolia1.PlantaMed1986,52:340.69.BudavariSONM,SmithA,HechelmanP,KinnearyLEds:TheMerckIndexedn12thed.NewYork:ChapmanandHall;1997.70.BakkaliF,AverbeckS,AverbeckD,IdaomarM:Biologicaleffectsofessentialoils--areview.FoodChemToxicol2008,46:446-475.71.UlteeA,KetsEP,SmidEJ:Mechanismsofactionofcarvacrolonthefood-bornepathogenBacilluscereus.ApplEnvironMicrobiol1999,65:4606-4610.72.NostroA,BlancoAR,CannatelliMA,EneaV,FlaminiG,MorelliI,SudanoRoccaroA,AlonzoV:Susceptibilityofmethicillin-resistantstaphylococcitooreganoessentialoil,carvacrolandthymol.FEMSMicrobiolLett2004,230:191-195.73.GillAO,HolleyRA:DisruptionofEscherichiacoli,ListeriamonocytogenesandLactobacillussakeicellularmembranesbyplantoilaromatics.IntJFoodMicrobiol2006,108:1-9.74.UlteeA,BennikMH,MoezelaarR:Thephenolichydroxylgroupofcarvacrolisessentialforactionagainstthefood-bornepathogenBacilluscereus.ApplEnvironMicrobiol2002,68:1561-1568.75.ZeytinogluMAS,OzturkY,BaserKHCInhibitoryeffectsofcarvacrolonDMBAinducedpulmonarytumorigenesisinrats.Actapharmaceuticaturcica1998,40:6.76.ZeytinogluH,IncesuZ,BaserKH:InhibitionofDNAsynthesisbycarvacrolinmousemyoblastcellsbearingahumanN-RASoncogene.Phytomedicine2003,10:292-299.77.SonD-JPY-H,KimY-M,ChungM-H,LeeH-S:AntiplateletactivityofThujopsisdolabratavar.hondai-derivedcomponentagainstplateletaggregation.Journalofmicrobiologyandbiotechnology2005,15:3.78.PrietoJM,IacopiniP,CioniP,ChericoniS:InvitroactivityoftheessentialoilsofOriganumvulgare,Saturejamontanaandtheirmainconstituentsinperoxynitrite-inducedoxidativeprocesses.FoodChemistry2007,104:889-895.79.KarpouhtsisI,PardaliE,FeggouE,KokkiniS,ScourasZG,Mavragani-TsipidouP:InsecticidalandGenotoxicActivitiesofOreganoEssentialOils.JournalofAgriculturalandFoodChemistry1998,46:1111-1115.80.UyanogluM,CanbekM,AralE,HusnuCanBaserK:Effectsofcarvacrolupontheliverofratsundergoingpartialhepatectomy.Phytomedicine2008,15:226-229.81.MagyarJ,SzentandrassyN,BanyaszT,FulopL,VarroA,NanasiPP:Effectsofterpenoidphenolderivativesoncalciumcurrentincanineandhumanventricularcardiomyocytes.EurJPharmacol2004,487:29-36.82.DiPasquaR,BettsG,HoskinsN,EdwardsM,ErcoliniD,MaurielloG:Membranetoxicityofantimicrobialcompoundsfromessentialoils.JAgricFoodChem2007,55:4863-4870.83.CristaniM,D'ArrigoM,MandalariG,CastelliF,SarpietroMG,MicieliD,VenutiV,BisignanoG,SaijaA,TrombettaD:Interactionoffourmonoterpenescontainedinessentialoilswithmodelmembranes:implicationsfortheirantibacterialactivity.JAgricFoodChem2007,55:6300-6308.  國圖紙本論文 推文 網路書籤 推薦 評分 引用網址 轉寄                                                                                                                                                                                                                    top 相關論文 相關期刊 熱門點閱論文 無相關論文   無相關期刊   1. LactobacillusplantarumPS128對小鼠情緒行為之影響 2. 探討台灣馬桑莖部沉澱物對單純皰疹病毒第一型複製的抑制作用 3. 探討茶多酚類化合物對於第一型單純皰疹病毒感染口腔上皮細胞的影響 4. 單純皰疹病毒感染中魚腥草抗病毒作用之機制及miR-27a*所扮演之角色 5. 探討肝醣合成酶激酶-3於第一型單純皰疹病毒感染中的角色 6. 以過敏小鼠動物模式探討乳酸桿菌YM0547的免疫調節活性 7. 植物精油對白色念珠菌生物膜之影響 8. β-細辛醚對於蝕骨細胞分化以及骨質蝕損之抑制作用 9. 建立以DOI誘導類妥瑞行為之動物模式並篩選具有改善妥瑞行為的乳酸菌及中草藥 10. 植物化學成分抑制脂肪細胞分化之研究 11. 當歸與天竺葵精油及其成分抗發炎作用機制研究 12. 甘松精油對於突變型鏈球菌致病能力之影響 13. 山茱萸發酵液之活性物質及抗氧化性之探討 14. 利用非侵入性方法研究精油對大腦認知功能的影響 15. 乳香與沒藥精油~對於失眠應用     簡易查詢 | 進階查詢 | 熱門排行 | 我的研究室